Image Stack Open

An image stack combines multiple images into a single image file or collection.

The images that make up a single stack can be stored in a single multi-image file or in individual files.

There are two formats for the multi-image file:

• The PIC format is a BioRad proprietary format.
• The TIF format is a non-proprietary format that also supports multiple images in a single file. Fluoview files are a type of TIF file.

Images stored in individual files can be loaded using the Image Stack Open command.

Some image stacks contain scaling information that Neurolucida uses to determine the distances between images.

If your stacks don't have scaling information, you can enter the distances to use manually via the Image Scaling dialog box.